One of the benefits of BINSEQ is that we don’t need to keep separate files for the different read pairs. BINSEQ supports R1 and R2 pairing in the same file.
To create a paired file we can pass two files to the bqtools encode command:
By default, bqtools will autodetermine the output path from the sample names (in this case: fastq/sample1.vbq) - but you can override this with the -o/--output option.