If no further pruning is needed, we can just use the alignment from the previous step to build a phylogeny. There are various tools for doing this, such as VeryFastTree and ggtree. We will use a very simple Python script that makes use of the BioPython library, that runs quickly in the browser. It will read the alignment, create a tree, and print it in terminal. Run it executing the following:

This shows that some of the assemblies are more closely related than the others.