Call variants
Now that we have reads aligned to the reference genome, let’s call variants using bcftools. Output the variants to the file variants.bcf.
Hint: Check out the bcftools section of the bowtie2 tutorial for an example of how to run bcftools.
variants.bcf existsvariants.bcf contains the variantsvariants.bcf.csi is the index file of variants.bcf obtained with bcftools index